Abstract
A sensitive and versatile detection scheme based on quantum dot immobilisation on a solid support through bio-orthogonal PCR amplification and labelling has been developed for detection and quantification of gene targets in complex DNA mixtures.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies / chemistry
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Biological Assay*
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Biotin / chemistry
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DNA / chemistry*
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DNA Primers / chemistry
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Digoxigenin / chemistry
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Histidine Decarboxylase / analysis
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Histidine Decarboxylase / genetics
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Humans
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Leukocytes / cytology
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Leukocytes / metabolism
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Plasmids
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Point-of-Care Systems
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Polymerase Chain Reaction / methods*
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Quantum Dots
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Sensitivity and Specificity
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Spectrometry, Fluorescence
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Staining and Labeling / methods*
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Streptavidin / chemistry
Substances
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Antibodies
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DNA Primers
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Biotin
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DNA
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Streptavidin
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Histidine Decarboxylase
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Digoxigenin