This eBook is a comprehensive, crucial resource for investigators optimizing assays to evaluate collections of molecules with the overall goal of developing probes that modulate the activity of biological targets, pathways or cellular phenotypes. Such probes might be candidates for further optimization and investigation in drug discovery and development.
Originally written as a guide for therapeutic project teams within a major pharmaceutical company, this manual has been adapted to provide guidelines for scientists in academic, non-profit, government and industrial research laboratories to develop assay formats compatible with
Descriptions of assay formats that are compatible with
Selection and development of optimal assay reagents
Optimizations and troubleshooting for assay protocols with respect to sensitivity, dynamic range, signal intensity and stability
Adaptations of assays for automation and scaling to microtiter plate formats
Instrumentation
Sources of assay artifacts and interferences
Statistical validation of assay performance parameters
Secondary assays for chemical probe validation and SAR refinement
Data standards for reporting the results of screening and SAR assays
In vivo assay development and validation
Assay development and validation for siRNA-based high-throughput screens
The
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