Abstract
Sex-chromosome dosage compensation requires selective identification of X chromatin. How this occurs is not fully understood. We show that small interfering RNA (siRNA) mutations enhance the lethality of Drosophila males deficient in X recognition and partially rescue females that inappropriately dosage-compensate. Our findings are consistent with a role for siRNA in selective recognition of X chromatin.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Dosage Compensation, Genetic / genetics*
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Drosophila Proteins / deficiency
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Drosophila Proteins / genetics
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Drosophila melanogaster / genetics*
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Female
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Gene Knockdown Techniques
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Male
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Mutation
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RNA, Small Interfering / genetics*
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RNA-Binding Proteins / genetics
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Transcription Factors / deficiency
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Transcription Factors / genetics
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X Chromosome / genetics*
Substances
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Drosophila Proteins
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Pabp2 protein, Drosophila
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RNA, Small Interfering
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RNA-Binding Proteins
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Transcription Factors
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roX1 protein, Drosophila