Active case detection with pooled real-time PCR to eliminate malaria in Trat province, Thailand

Am J Trop Med Hyg. 2012 May;86(5):789-91. doi: 10.4269/ajtmh.2012.11-0617.

Abstract

We conducted contact tracing and high-risk group screening using pooled real-time polymerase chain reaction (PCR) to support malaria elimination in Thailand. PCR detected more Plasmodium infections than the local and expert microscopists. High-throughput pooling technique reduced costs and allowed prompt reporting of results.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Contact Tracing*
  • DNA, Protozoan / genetics
  • DNA, Protozoan / isolation & purification*
  • Female
  • Humans
  • Malaria / diagnosis*
  • Malaria / epidemiology*
  • Male
  • Middle Aged
  • Real-Time Polymerase Chain Reaction / methods*
  • Thailand / epidemiology
  • Young Adult

Substances

  • DNA, Protozoan