Abstract
A simple, efficient and economical method for the recovery of P(3HB-co-3HHx) was developed using various chemicals and parameters. The initial content of P(3HB-co-3HHx) in bacterial cells was 50-60 wt%, whereas the monomer composition of 3HHx used in this experiments was 3-5 mol%. It was found that sodium hydroxide (NaOH) was the most effective chemical for the recovery of biodegradable polymer. High polyhydroxyalkanoate purity and recovery yield both in the range of 80-90 wt% were obtained when 10-30 mg/ml of cells were incubated in NaOH at the concentration of 0.1 M for 60-180 min at 30 °C and polished using 20 % (v/v) of ethanol.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
3-Hydroxybutyric Acid / biosynthesis
-
3-Hydroxybutyric Acid / chemistry*
-
3-Hydroxybutyric Acid / isolation & purification*
-
Acyltransferases / genetics
-
Caproates / chemistry*
-
Caproates / isolation & purification*
-
Chemical Fractionation / methods*
-
Cupriavidus necator / cytology
-
Cupriavidus necator / genetics*
-
Cupriavidus necator / metabolism
-
DNA, Recombinant / genetics*
-
Green Chemistry Technology / economics
-
Green Chemistry Technology / methods*
-
Palm Oil
-
Plant Oils / metabolism
-
Plasmids / genetics
-
Sodium Hydroxide / chemistry
-
Solvents / chemistry
-
Water / chemistry
Substances
-
Caproates
-
DNA, Recombinant
-
Plant Oils
-
Solvents
-
poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
-
Water
-
Sodium Hydroxide
-
Palm Oil
-
Acyltransferases
-
poly(3-hydroxyalkanoic acid) synthase
-
3-Hydroxybutyric Acid