Abstract
A PECAM-1 specific PET imaging agent, PECAM-1-Ab-DOTA-(64)Cu, was synthesized by conjugating the anti-mouse PECAM-1 antibody with 2,2',2",2"'-(1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrayl)tetraacetic acid (DOTA) and subsequent labeling with (64)Cu. Positron Emission Tomography (PET) was successfully performed in a mouse model of myocardial infarction (MI) induced by an ischemia/reperfusion (I/R) injury, indicating the elevated expression of PECAM-1.
Published by Elsevier Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / chemistry
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Chromatography, High Pressure Liquid
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Copper Radioisotopes
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Gadolinium / chemistry
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Gene Expression
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Heterocyclic Compounds, 1-Ring / chemistry
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Immunoconjugates / chemistry*
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Immunoconjugates / pharmacokinetics
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Male
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Mice
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Mice, Inbred C57BL
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Myocardial Infarction / diagnostic imaging
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Myocardial Infarction / etiology
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Myocardial Infarction / pathology*
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Myocardial Reperfusion Injury / complications
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Myocardial Reperfusion Injury / diagnostic imaging
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Myocardial Reperfusion Injury / pathology*
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Platelet Endothelial Cell Adhesion Molecule-1 / genetics
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Platelet Endothelial Cell Adhesion Molecule-1 / immunology
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Positron-Emission Tomography / methods*
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Radioimmunoassay
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Radiopharmaceuticals / chemistry*
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Radiopharmaceuticals / pharmacokinetics
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Staining and Labeling / methods
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Tissue Distribution
Substances
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Antibodies
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Copper Radioisotopes
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Heterocyclic Compounds, 1-Ring
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Immunoconjugates
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Platelet Endothelial Cell Adhesion Molecule-1
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Radiopharmaceuticals
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1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid
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Gadolinium