Plasma separation by centrifugation and subsequent plasma filtration: impact on survival in a pig model of sepsis

Ther Apher Dial. 2012 Jun;16(3):205-12. doi: 10.1111/j.1744-9987.2011.01055.x. Epub 2012 Mar 5.

Abstract

The impact on survival of a combination of plasma separation by centrifugation and subsequent plasma filtration was tested in a bacterial sepsis model in pigs. In this animal study 19 pigs were included. Groups II and III received an intravenous lethal dose of live Staphylococcus aureus over 1 h; group I received saline (non-septic control--NC). Groups I and II were treated by an extracorporeal circuit consisting of online centrifugation and subsequent plasma filtration (group II: treated group--TG) for 4 h; group III had no specific treatment (septic control, SC). The observation time was 7 days. All animals of group I (NC) and group II (TG) survived, while all animals of group III (SC) died during the observation time. Extracorporeal therapy with online centrifugation and plasma filtration significantly improved survival in a pig model of sepsis. Further studies with this approach are encouraged.

MeSH terms

  • Animals
  • Biomarkers / analysis
  • Centrifugation
  • Cytokines / analysis
  • Disease Models, Animal
  • Extracorporeal Circulation
  • Female
  • Filtration / methods
  • Kidney Function Tests
  • Liver Function Tests
  • Plasmapheresis / methods
  • Prospective Studies
  • Sepsis / blood*
  • Sepsis / microbiology
  • Sepsis / therapy*
  • Staphylococcal Infections / blood*
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / therapy*
  • Statistics, Nonparametric
  • Swine

Substances

  • Biomarkers
  • Cytokines