Manual microdissection technique in a case of subcutaneous panniculitis-like T-cell lymphoma: a case report and review

J Cutan Pathol. 2012 Aug;39(8):769-72. doi: 10.1111/j.1600-0560.2012.01921.x. Epub 2012 May 22.

Abstract

Demonstration of T-cell receptor gene monoclonality often plays an important role in the diagnosis of T-cell lymphoma. When a test to detect monoclonality is performed on whole tissue sections, the presence of a reactive lymphocyte population may reduce sensitivity. This may be especially true for early or borderline cases of lymphoma. Microdissection techniques may be utilized to more readily identify a clonal population of lymphocytes. Subcutaneous panniculitis-like T-cell lymphoma represents a cutaneous lymphoid neoplasm whose clinical course may vary from an indolent, waxing and waning course to an aggressive course resulting in death. We report the first case of a microdissection technique used to facilitate diagnosing a case of subcutaneous panniculitis-like T-cell lympoma.

Publication types

  • Case Reports
  • Review

MeSH terms

  • Clone Cells
  • DNA, Neoplasm / analysis
  • Female
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / genetics
  • Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor / genetics
  • Humans
  • Lymphoma, T-Cell, Cutaneous / diagnosis*
  • Lymphoma, T-Cell, Cutaneous / genetics
  • Lymphoma, T-Cell, Cutaneous / surgery
  • Microdissection / methods*
  • Middle Aged
  • Panniculitis / genetics
  • Panniculitis / pathology*
  • Panniculitis / surgery
  • Pathology, Molecular / methods
  • Skin Neoplasms / diagnosis*
  • Skin Neoplasms / genetics
  • Skin Neoplasms / surgery
  • Subcutaneous Tissue / pathology
  • Subcutaneous Tissue / surgery

Substances

  • DNA, Neoplasm