Study of the effect of a glycosaminoglycan-peptide complex on the degradative enzyme activities in human osteoarthritic cartilage

E Vignon; A Martin; P Mathieu; T Conrozier; P Louisot; M Richard

doi:10.1007/BF02114400

Study of the effect of a glycosaminoglycan-peptide complex on the degradative enzyme activities in human osteoarthritic cartilage

Clin Rheumatol. 1990 Sep;9(3):383-8. doi: 10.1007/BF02114400.

Authors

E Vignon¹, A Martin, P Mathieu, T Conrozier, P Louisot, M Richard

Affiliation

¹ Laboratoire d'Exploration Articulaire, Hôpital Edouard Herriot, Lyon, France.

PMID: 2261738
DOI: 10.1007/BF02114400

Abstract

The in vitro collagenolytic and proteoglycanasic activity from human fibrillated osteoarthritic cartilage was determined using labelled proteoglycans and type II collagen as substrates. In vitro, a glycosaminoglycan-peptide complex (GP-C Rumalon) induced a dose-dependent inhibition of both collagenolytic and proteoglycanasic activities while sodium salicylate and indomethacin had only a weak suppressive effect on proteoglycanase. Phospholipase A2 activity was unmodified by GP-C suggesting that the effect of the drug on degradative enzymes was unrelated to prostaglandin formation.

MeSH terms

Aged
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Cartilage, Articular / enzymology*
Collagen / metabolism
Dose-Response Relationship, Drug
Endopeptidases / metabolism
Female
Femur Head / enzymology
Humans
Male
Metalloendopeptidases*
Middle Aged
Osteoarthritis / enzymology*
Phospholipases A / metabolism
Phospholipases A2
Tissue Extracts / pharmacology*

Substances

Anti-Inflammatory Agents, Non-Steroidal
Tissue Extracts
Rumalon
Collagen
Phospholipases A
Phospholipases A2
Endopeptidases
proteoglycan-degrading metalloendopeptidases
Metalloendopeptidases