Abstract
Histone octamers are the basic building blocks of chromatin and are platforms for diverse genetic mechanisms. We report a simple method for preparing recombinant histone octamers by overexpressing all four histones from a single polycistronic vector followed by standard chromatography under native conditions. This approach reduces the time needed for the octamer preparation to a single day and should be applicable to making a variety of unmodified and modified histone octamers.
Copyright © 2012 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria
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Bacterial Proteins / genetics*
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Bacterial Proteins / isolation & purification
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Bacterial Proteins / metabolism
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Chromatin / genetics*
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Chromatin / metabolism
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Chromatography, Gel
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Electrophoresis, Polyacrylamide Gel
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Gene Expression
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Genetic Vectors / chemistry
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Genetic Vectors / genetics*
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Histones / genetics*
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Histones / isolation & purification
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Histones / metabolism
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Nucleosomes / genetics
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Nucleosomes / metabolism
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Protein Isoforms / genetics
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Protein Isoforms / isolation & purification
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Protein Isoforms / metabolism
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Protein Multimerization
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
Substances
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Bacterial Proteins
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Chromatin
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Histones
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Nucleosomes
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Protein Isoforms
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Recombinant Proteins