Conformations of helical Aib peptides containing a pair of L-amino acid and D-amino acid

J Pept Sci. 2012 Jul;18(7):466-75. doi: 10.1002/psc.2418. Epub 2012 May 22.

Abstract

A pair of L-leucine (L-Leu) and D-leucine (D-Leu) was incorporated into a-aminoisobutyric acid (Aib) peptide segments. Thedominant conformations of four hexapeptides, Boc-L-Leu-Aib-Aib-Aib-Aib-L-Leu-OMe (1a), Boc-D-Leu-Aib-Aib-Aib-Aib-L-Leu-OMe(1b), Boc-Aib-Aib-L-Leu-L-Leu-Aib-Aib-OMe (2a), and Boc-Aib-Aib-D-Leu-L-Leu-Aib-Aib-OMe (2b), were investigated by IR,¹H NMR, CD spectra, and X-ray crystallographic analysis. All peptides 1a,b and 2a,b formed 3₁₀-helical structures in solution. X-ray crystallographic analysis revealed that right-handed (P) 3₁₀-helices were present in 1a and 1b and a mixture of right-handed(P) and left-handed (M) 3₁₀-helices was present in 2b in their crystalline states.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoisobutyric Acids / chemistry*
  • Crystallography, X-Ray
  • Leucine / chemistry*
  • Models, Molecular
  • Molecular Conformation
  • Oligopeptides / chemical synthesis
  • Oligopeptides / chemistry*
  • Stereoisomerism

Substances

  • Aminoisobutyric Acids
  • Oligopeptides
  • 2-aminoisobutyric acid
  • Leucine