Abstract
Herpes simplex virus type 1 (HSV-1) triplex is a complex of three protein subunits, consisting of two copies of VP23 and one copy of VP19C. Here, we identified a non-classical NLS of VP19C between aa 50 and 61, and the nuclear import of VP19C was mediated by RanGTP and importin β1-, but not importin α5-, dependent pathway. Additionally, recombinant virus harbouring this NLS mutation (NLSm) replicates less efficiently as wild-type. These data strongly suggested that the nuclear import of VP19C is required for efficient HSV-1 production.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus
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Amino Acid Sequence
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Animals
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Capsid Proteins / chemistry*
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Capsid Proteins / genetics
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Capsid Proteins / metabolism*
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Cell Line
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Cell Nucleus / genetics
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Cell Nucleus / metabolism*
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Herpes Simplex / metabolism
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Herpes Simplex / virology*
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Herpesvirus 1, Human / chemistry
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Herpesvirus 1, Human / genetics
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Herpesvirus 1, Human / physiology*
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Humans
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Molecular Sequence Data
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Nuclear Localization Signals*
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Virus Assembly
Substances
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Capsid Proteins
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Nuclear Localization Signals
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VP19 protein, Human herpesvirus 1