Abstract
The accumulation of plasma advanced oxidation protein products (AOPPs) is prevalent in chronic kidney disease. We previously showed that accumulation of AOPPs resulted in podocyte apoptosis and their deletion by a cascade of signaling events coupled with intracellular oxidative stress. The transmembrane receptor that specifically transmits the AOPPs' signals to elicit cellular activity, however, remains unknown. Using co-immunoprecipitation and immunofluorescence, we found that AOPPs colocalized and interacted with the receptor of advanced glycation end products (RAGE) on podocytes. Blocking RAGE by anti-RAGE immunoglobulin G or its silencing by siRNA significantly protected podocytes from AOPPs-induced apoptosis both in vitro and in vivo and ameliorated albuminuria in AOPPs-challenged mice. AOPPs-induced activation of nicotinamide adenine dinucleotide phosphate oxidase and the excessive generation of intracellular superoxide were largely inhibited by anti-RAGE immunoglobulin G or RAGE siRNA. Moreover, blockade of RAGE decreased the activation of the p53/Bax/caspase-dependent proapoptotic pathway induced by AOPPs. Thus, AOPPs interact with RAGE to induce podocyte apoptosis and this, in part, may contribute to the progression of chronic kidney disease.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Advanced Oxidation Protein Products / administration & dosage
-
Advanced Oxidation Protein Products / metabolism*
-
Albuminuria / metabolism
-
Albuminuria / prevention & control
-
Animals
-
Apoptosis*
-
Caspase 3 / metabolism
-
Cell Line
-
Disease Progression
-
Enzyme Activation
-
Fluorescent Antibody Technique
-
Immunoglobulin G / administration & dosage
-
Immunoprecipitation
-
Injections, Intravenous
-
Male
-
Mice
-
Mice, Inbred BALB C
-
Mice, Inbred C57BL
-
NADPH Oxidases / metabolism
-
Podocytes / immunology
-
Podocytes / metabolism*
-
Podocytes / pathology
-
RNA Interference
-
Receptor for Advanced Glycation End Products
-
Receptors, Immunologic / genetics
-
Receptors, Immunologic / immunology
-
Receptors, Immunologic / metabolism*
-
Renal Insufficiency, Chronic / immunology
-
Renal Insufficiency, Chronic / metabolism*
-
Renal Insufficiency, Chronic / pathology
-
Renal Insufficiency, Chronic / prevention & control
-
Signal Transduction
-
Superoxides / metabolism
-
Time Factors
-
Transfection
-
Tumor Suppressor Protein p53 / metabolism
-
bcl-2-Associated X Protein / metabolism
Substances
-
Advanced Oxidation Protein Products
-
Bax protein, mouse
-
Immunoglobulin G
-
Receptor for Advanced Glycation End Products
-
Receptors, Immunologic
-
Tumor Suppressor Protein p53
-
bcl-2-Associated X Protein
-
Superoxides
-
NADPH Oxidases
-
Casp3 protein, mouse
-
Caspase 3