Climacteric lowers plasma levels of platelet-derived microparticles: a pilot study in pre- versus postmenopausal women

Acta Haematol. 2012;128(1):53-9. doi: 10.1159/000337327. Epub 2012 May 23.

Abstract

Background: Climacteric increases the risk of thrombotic events by alteration of plasmatic coagulation. Up to now, less is known about changes in platelet- (PMP) and endothelial cell-derived microparticles (EMP).

Methods: In this prospective study, plasma levels of microparticles (MP) were compared in 21 premenopausal and 19 postmenopausal women.

Results: No altered numbers of total MP or EMP were measured within the study groups. However, the plasma values of CD61-exposing MP from platelets/megakaryocytes were higher in premenopausal women (5,364 × 10(6)/l, range 4,384-17,167) as compared to postmenopausal women (3,808 × 10(6)/l, range 2,009-8,850; p = 0.020). This differentiation was also significant for the subgroup of premenopausal women without hormonal contraceptives (5,364 × 10(6)/l, range 4,223-15,916; p = 0.047; n = 15). Furthermore, in premenopausal women, higher plasma levels of PMP exposing CD62P were also present as compared to postmenopausal women (288 × 10(6)/l, range 139-462, vs. 121 × 10(6)/l, range 74-284; p = 0.024). This difference was also true for CD63+ PMP levels (281 × 10(6)/l, range 182-551, vs. 137 × 10(6)/l, range 64-432; p = 0.015).

Conclusion: Climacteric lowers the level of PMP but has no impact on the number of EMP in women. These data suggest that PMP and EMP do not play a significant role in enhancing the risk of thrombotic events in healthy, postmenopausal women.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Blood Platelets / metabolism*
  • Cell-Derived Microparticles / metabolism*
  • Climacteric
  • Endothelial Cells / metabolism*
  • Female
  • Flow Cytometry
  • Humans
  • Integrin beta3 / metabolism
  • Megakaryocytes / metabolism
  • P-Selectin / metabolism
  • Pilot Projects
  • Postmenopause
  • Premenopause
  • Tetraspanin 30 / metabolism
  • Young Adult

Substances

  • Integrin beta3
  • P-Selectin
  • Tetraspanin 30