Expression of CCR7 and CD45RA in CD4+ and CD8+ subsets in cerebrospinal fluid of 134 patients with inflammatory and non-inflammatory neurological diseases

J Neuroimmunol. 2012 Aug 15;249(1-2):86-92. doi: 10.1016/j.jneuroim.2012.04.017. Epub 2012 May 24.

Abstract

We investigated CD45RA and CCR7 expression in CD4+ and CD8+ subsets of cerebrospinal fluid (CSF) lymphocytes, both immediately ex vivo and after stimulation, from 134 patients with a variety of inflammatory and non-inflammatory neurological diseases. Most inflammatory diseases had a higher CD4+:CD8+ ratio and higher percentage of effector memory T cells (T(EM)) than non-inflammatory controls, excluding active infection. Moreover, we found that patients with highly elevated cell counts in the CSF tended to have a lower percentage of central memory T cells (T(CM)) than patients with low or absent pleocytosis, with a concomitant increase in T(EM). We also found that samples with elevated IgG index or presence of oligoclonal bands had a significantly higher CD4+:CD8+ ratio than normal samples, consistent with increased CD4+ help for intrathecal IgG synthesis by B cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism
  • Central Nervous System Diseases / cerebrospinal fluid*
  • Central Nervous System Diseases / immunology
  • Central Nervous System Diseases / metabolism
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Immunoglobulin G / cerebrospinal fluid
  • Infant
  • Inflammation / cerebrospinal fluid
  • Inflammation / immunology
  • Inflammation / metabolism
  • Leukocyte Common Antigens / biosynthesis
  • Leukocyte Common Antigens / cerebrospinal fluid
  • Leukocyte Common Antigens / immunology
  • Male
  • Middle Aged
  • Receptors, CCR7 / analysis
  • Receptors, CCR7 / biosynthesis
  • Receptors, CCR7 / immunology
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocyte Subsets / metabolism
  • Young Adult

Substances

  • CCR7 protein, human
  • Immunoglobulin G
  • Receptors, CCR7
  • Leukocyte Common Antigens