Abstract
Glucose catabolite repression of tetralin catabolic genes in Rhodococcus sp. strain TFB was shown to be exerted by a protein homologous to transcriptional regulators of the cyclic AMP receptor (CRP)-FNR family. The protein was detected bound to putative CRP-like boxes localized at the promoters of the thnA1 and thnS genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Catabolite Repression / genetics
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Catabolite Repression / physiology*
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Cyclic AMP Receptor Protein / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Electroporation
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Glucose / metabolism*
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Green Fluorescent Proteins
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Mutagenesis
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Plasmids / genetics
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Polymerase Chain Reaction
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Promoter Regions, Genetic / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Rhodococcus / metabolism
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Rhodococcus / physiology*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Tetrahydronaphthalenes / metabolism*
Substances
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Bacterial Proteins
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Cyclic AMP Receptor Protein
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Tetrahydronaphthalenes
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Green Fluorescent Proteins
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tetralin
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Glucose