Discrepancy between minimal inhibitory concentration to enrofloxacin and mutations present in the quinolone-resistance determining regions of Mycoplasma gallisepticum field strains

I Lysnyansky; I Gerchman; S Levisohn; I Mikula; A Feberwee; N M Ferguson; A H Noormohammadi; J Spergser; H M Windsor

doi:10.1016/j.vetmic.2012.05.002

Discrepancy between minimal inhibitory concentration to enrofloxacin and mutations present in the quinolone-resistance determining regions of Mycoplasma gallisepticum field strains

Vet Microbiol. 2012 Nov 9;160(1-2):222-6. doi: 10.1016/j.vetmic.2012.05.002. Epub 2012 May 15.

Authors

I Lysnyansky¹, I Gerchman, S Levisohn, I Mikula, A Feberwee, N M Ferguson, A H Noormohammadi, J Spergser, H M Windsor

Affiliation

¹ Mycoplasma Unit, Kimron Veterinary Institute, Bet Dagan, Israel. [email protected]

PMID: 22655973
DOI: 10.1016/j.vetmic.2012.05.002

Abstract

Molecular characterization of the quinolone-resistance determining regions (QRDRs) of DNA gyrase and topoisomerase IV in 93 Mycoplasma gallisepticum field strains isolated in different geographic regions revealed discrepancies between minimal inhibitory concentration values and presence of amino acid substitutions within the QRDRs of GyrA and ParC in 9/93 (10%) strains. This may delimitate applicability of a gene-based assay to detect fluoroquinolone resistance in this avian pathogen.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution
Animals
Birds
DNA Gyrase / genetics
DNA Topoisomerase IV / chemistry
DNA Topoisomerase IV / genetics
Enrofloxacin
Fluoroquinolones / pharmacology*
Microbial Sensitivity Tests
Mutation*
Mycoplasma Infections / microbiology
Mycoplasma gallisepticum / drug effects*
Mycoplasma gallisepticum / genetics*
Mycoplasma gallisepticum / isolation & purification
Poultry Diseases / microbiology
Quinolones / pharmacology

Substances

Fluoroquinolones
Quinolones
Enrofloxacin
DNA Topoisomerase IV
DNA Gyrase