Efficient quantification of HIV-1 in heparin plasma spiked with cultured HIV-1 by the Roche Cobas TaqMan and Abbott RealTime HIV-1 tests

J Clin Microbiol. 2012 Aug;50(8):2804-6. doi: 10.1128/JCM.00706-12. Epub 2012 Jun 6.

Abstract

The current automated real-time HIV-1 viral load assays, the Roche Cobas AmpliPrep/Cobas TaqMan test and the Abbott RealTime test, are FDA cleared for use with EDTA plasma. We show that both real-time reverse transcription-PCR (RT-PCR) tests reliably quantify HIV-1 RNA in heparin plasma specimens spiked with HIV-1 isolate MN.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anticoagulants / metabolism
  • Automation / methods
  • HIV-1 / isolation & purification*
  • Heparin / metabolism
  • Humans
  • Plasma / virology*
  • Real-Time Polymerase Chain Reaction / methods*
  • Specimen Handling / methods*
  • Viral Load / methods*

Substances

  • Anticoagulants
  • Heparin