Abstract
The stress response of eukaryotic cells often causes an attenuation of bulk translation activity and the accumulation of non-translating mRNAs into cytoplasmic mRNP (messenger ribonucleoprotein) granules termed cytoplasmic P-bodies (processing bodies) and SGs (stress granules). We examined effects of acidic stress on the formation of mRNP granules compared with other forms of stress such as glucose deprivation and a high Ca²⁺ level in Saccharomyces cerevisiae. Treatment with lactic acid clearly caused the formation of P-bodies, but not SGs, and also caused an attenuation of translation initiation, albeit to a lesser extent than glucose depletion. P-body formation was also induced by hydrochloric acid and sulfuric acid. However, lactic acid in SD (synthetic dextrose) medium with a pH greater than 3.0, propionic acid and acetic acid did not induce P-body formation. The results of the present study suggest that the assembly of yeast P-bodies can be induced by external conditions with a low pH and the threshold was around pH 2.5. The P-body formation upon acidic stress required Scd6 (suppressor of clathrin deficiency 6), a component of P-bodies, indicating that P-bodies induced by acidic stress have rules of assembly different from those induced by glucose deprivation or high Ca²⁺ levels.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Carboxylic Acids / pharmacology*
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Clathrin / metabolism
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Cytoplasmic Granules / drug effects*
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Cytoplasmic Granules / metabolism
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Cytoskeletal Proteins / biosynthesis
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Cytoskeletal Proteins / genetics
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Cytoskeletal Proteins / metabolism
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Food Preservatives / pharmacology*
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Fungicides, Industrial / pharmacology*
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Hydrogen-Ion Concentration
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Microbial Viability / drug effects
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Microscopy, Fluorescence
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Osmolar Concentration
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Polyribosomes / drug effects
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Polyribosomes / metabolism
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Protein Biosynthesis / drug effects
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RNA Cap-Binding Proteins / biosynthesis
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RNA Cap-Binding Proteins / genetics
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RNA Cap-Binding Proteins / metabolism
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RNA, Fungal / metabolism
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RNA, Messenger / metabolism
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Ribonucleoproteins / metabolism
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Saccharomyces cerevisiae / drug effects*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / biosynthesis*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Stress, Physiological
Substances
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Carboxylic Acids
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Clathrin
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Cytoskeletal Proteins
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Food Preservatives
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Fungicides, Industrial
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RNA Cap-Binding Proteins
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RNA, Fungal
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RNA, Messenger
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Recombinant Proteins
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Ribonucleoproteins
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Saccharomyces cerevisiae Proteins