Dietary fish oil substitution alters the eicosanoid profile in ankle joints of mice during Lyme infection

J Nutr. 2012 Aug;142(8):1582-9. doi: 10.3945/jn.112.157883. Epub 2012 Jun 13.

Abstract

Dietary ingestion of (n-3) PUFA alters the production of eicosanoids and can suppress chronic inflammatory and autoimmune diseases. The extent of changes in eicosanoid production during an infection of mice fed a diet high in (n-3) PUFA, however, has not, to our knowledge, been reported. We fed mice a diet containing either 18% by weight soybean oil (SO) or a mixture with fish oil (FO), FO:SO (4:1 ratio), for 2 wk and then infected them with Borrelia burgdorferi. We used an MS-based lipidomics approach and quantified changes in eicosanoid production during Lyme arthritis development over 21 d. B. burgdorferi infection induced a robust production of prostanoids, mono-hydroxylated metabolites, and epoxide-containing metabolites, with 103 eicosanoids detected of the 139 monitored. In addition to temporal and compositional changes in the eicosanoid profile, dietary FO substitution increased the accumulation of 15-deoxy PGJ(2), an antiinflammatory metabolite derived from arachidonic acid. Chiral analysis of the mono-hydroxylated metabolites revealed they were generated from primarily nonenzymatic mechanisms. Although dietary FO substitution reduced the production of inflammatory (n-6) fatty acid-derived eicosanoids, no change in the host inflammatory response or development of disease was detected.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animal Feed
  • Animals
  • Dietary Fats, Unsaturated / administration & dosage
  • Dietary Fats, Unsaturated / pharmacology*
  • Eicosanoids / metabolism*
  • Fatty Acids / blood
  • Fatty Acids / chemistry
  • Fatty Acids / metabolism
  • Female
  • Fish Oils / administration & dosage
  • Fish Oils / pharmacology*
  • Hindlimb
  • Hot Temperature
  • Joints / metabolism*
  • Joints / pathology
  • Liver / chemistry
  • Liver / metabolism
  • Lyme Disease / diet therapy*
  • Lyme Disease / metabolism*
  • Mice
  • Mice, Inbred C3H

Substances

  • Dietary Fats, Unsaturated
  • Eicosanoids
  • Fatty Acids
  • Fish Oils