A loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: application to a field survey

Agathe Nkouawa; Yasuhito Sako; Tiaoying Li; Xingwang Chen; Minoru Nakao; Tetsuya Yanagida; Munehiro Okamoto; Patrick Giraudoux; Francis Raoul; Kazuhiro Nakaya; Ning Xiao; Jiamin Qiu; Dongchuan Qiu; Philip S Craig; Akira Ito

doi:10.1016/j.parint.2012.06.001

A loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: application to a field survey

Parasitol Int. 2012 Dec;61(4):723-5. doi: 10.1016/j.parint.2012.06.001. Epub 2012 Jun 11.

Authors

Agathe Nkouawa¹, Yasuhito Sako, Tiaoying Li, Xingwang Chen, Minoru Nakao, Tetsuya Yanagida, Munehiro Okamoto, Patrick Giraudoux, Francis Raoul, Kazuhiro Nakaya, Ning Xiao, Jiamin Qiu, Dongchuan Qiu, Philip S Craig, Akira Ito

Affiliation

¹ Department of Parasitology, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510, Hokkaido, Japan.

PMID: 22698671
DOI: 10.1016/j.parint.2012.06.001

Abstract

In this study, we applied a loop-mediated isothermal amplification method for identification of human Taenia tapeworms in Tibetan communities in Sichuan, China. Out of 51 proglottids recovered from 35 carriers, 9, 1, and 41 samples were identified as Taenia solium, Taenia asiatica and Taenia saginata, respectively. Same results were obtained afterwards in the laboratory, except one sample. These results demonstrated that the LAMP method enabled rapid identification of parasites in the field surveys, which suggested that this method would contribute to the control of Taenia infections in endemic areas.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cestode Infections / epidemiology
Cestode Infections / parasitology*
Humans
Nucleic Acid Amplification Techniques / methods*
Species Specificity
Taenia / classification*
Taenia / genetics*
Tibet / epidemiology