Safe deprotection strategy for the tert-butyldimethylsilyl (TBS) group during RNA synthesis

Curr Protoc Nucleic Acid Chem. 2012 Jun:Chapter 3:Unit3.21. doi: 10.1002/0471142700.nc0321s49.

Abstract

This unit describes two protocols for the deprotection of 2'-O-TBS groups in oligoribonucleotides under mild conditions. Desilylation using ammonium fluoride is applicable to fully protected "RNA only" substrates and desilylation using potassium fluoride is applicable to "mixed RNA/non-RNA" substrates. Characterization of products is accomplished using LC/MS, RP HPLC and SAX HPLC.

MeSH terms

  • Ammonium Compounds
  • Chromatography, High Pressure Liquid
  • Fluorides / chemistry
  • Oligonucleotide Probes / chemical synthesis*
  • Organosilicon Compounds / chemistry*
  • Quaternary Ammonium Compounds / chemistry
  • RNA / chemical synthesis*

Substances

  • Ammonium Compounds
  • Oligonucleotide Probes
  • Organosilicon Compounds
  • Quaternary Ammonium Compounds
  • t-butyldimethylsilyl compounds
  • ammonium fluoride
  • RNA
  • Fluorides