Abstract
This unit describes two protocols for the deprotection of 2'-O-TBS groups in oligoribonucleotides under mild conditions. Desilylation using ammonium fluoride is applicable to fully protected "RNA only" substrates and desilylation using potassium fluoride is applicable to "mixed RNA/non-RNA" substrates. Characterization of products is accomplished using LC/MS, RP HPLC and SAX HPLC.
MeSH terms
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Ammonium Compounds
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Chromatography, High Pressure Liquid
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Fluorides / chemistry
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Oligonucleotide Probes / chemical synthesis*
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Organosilicon Compounds / chemistry*
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Quaternary Ammonium Compounds / chemistry
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RNA / chemical synthesis*
Substances
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Ammonium Compounds
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Oligonucleotide Probes
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Organosilicon Compounds
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Quaternary Ammonium Compounds
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t-butyldimethylsilyl compounds
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ammonium fluoride
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RNA
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Fluorides