Prediction of the stability of modified RNA duplexes based on deformability analysis: oligoribonucleotide derivatives modified with 2'-O-cyanoethyl-5-propynyl-2-thiouridine as a promising component

Yoshiaki Masaki; Ryuta Miyasaka; Kunihiro Hirai; Hirosuke Tsunoda; Akihiro Ohkubo; Kohji Seio; Mitsuo Sekine

doi:10.1039/c2cc33409g

Prediction of the stability of modified RNA duplexes based on deformability analysis: oligoribonucleotide derivatives modified with 2'-O-cyanoethyl-5-propynyl-2-thiouridine as a promising component

Chem Commun (Camb). 2012 Jul 25;48(58):7313-5. doi: 10.1039/c2cc33409g. Epub 2012 Jun 18.

Authors

Yoshiaki Masaki¹, Ryuta Miyasaka, Kunihiro Hirai, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Affiliation

¹ Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, Japan.

PMID: 22710854
DOI: 10.1039/c2cc33409g

Abstract

We describe a method to predict the stability of a modified RNA duplex. Ten unique modified RNA duplexes showed a linear relationship between the calculated and experimentally determined duplex stabilities.

MeSH terms

Nucleic Acid Conformation
Oligoribonucleotides / chemistry*
RNA / chemistry*
RNA Stability
Thiouridine / analogs & derivatives*
Thiouridine / chemistry
Transition Temperature

Substances

2-thiouridine
Oligoribonucleotides
Thiouridine
RNA