Evaluation of SNPs in miR-196-a2, miR-27a and miR-146a as risk factors of colorectal cancer

World J Gastroenterol. 2012 Jun 14;18(22):2827-31. doi: 10.3748/wjg.v18.i22.2827.

Abstract

Aim: To investigate whether selected single nucleotide polymorphisms (SNPs) in miR-196a2, miR-27a and miR-146a genes are associated with sporadic colorectal cancer (CRC).

Methods: In order to investigate the effect of these SNPs in CRC, we performed a case-control study of 197 cases of sporadic CRC and 212 cancer-free controls originating from the Central-European Caucasian population using TaqMan Real-Time polymerase chain reaction and allelic discrimination analysis.

Results: The genotype and allele frequencies of SNPs were compared between the cases and the controls. None of the performed analysis showed any statistically significant results.

Conclusion: Our data suggest a lack of association between rs11614913, rs895819 and rs2910164 and colorectal cancer risk in the Central-European Caucasian population, a population with an extremely high incidence of sporadic colorectal cancer.

Keywords: Association study; Colorectal cancer; MicroRNA; Single nucleotide polymorphism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / ethnology
  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Aged
  • Case-Control Studies
  • Chi-Square Distribution
  • Colorectal Neoplasms / ethnology
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / pathology
  • Czech Republic / epidemiology
  • Female
  • Gene Frequency
  • Genetic Predisposition to Disease
  • Humans
  • Incidence
  • Logistic Models
  • Male
  • MicroRNAs / genetics*
  • Middle Aged
  • Odds Ratio
  • Polymorphism, Single Nucleotide*
  • Real-Time Polymerase Chain Reaction
  • Risk Assessment
  • Risk Factors
  • White People / genetics

Substances

  • MIRN146 microRNA, human
  • MIRN196 microRNA, human
  • MIRN27 microRNA, human
  • MicroRNAs