Next generation deep sequencing and vaccine design: today and tomorrow

Fabio Luciani; Rowena A Bull; Andrew R Lloyd

doi:10.1016/j.tibtech.2012.05.005

Next generation deep sequencing and vaccine design: today and tomorrow

Trends Biotechnol. 2012 Sep;30(9):443-52. doi: 10.1016/j.tibtech.2012.05.005. Epub 2012 Jun 20.

Authors

Fabio Luciani¹, Rowena A Bull, Andrew R Lloyd

Affiliation

¹ Inflammation and Infection Research Centre, School of Medical Sciences, University of New South Wales, Sydney, Australia. [email protected]

Abstract

Next generation sequencing (NGS) technologies have redefined the modus operandi in both human and microbial genetics research, allowing the unprecedented generation of very large sequencing datasets on a short time scale and at affordable costs. Vaccine development research is rapidly taking full advantage of the advent of NGS. This review provides a concise summary of the current applications of NGS in relation to research seeking to develop vaccines for human infectious diseases, incorporating studies of both the pathogen and the host. We focus on rapidly mutating viral pathogens, which are major targets in current vaccine research. NGS is unraveling the complex dynamics of viral evolution and host responses against these viruses, thus contributing substantially to the likelihood of successful vaccine development.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Communicable Disease Control
Computational Biology / methods*
Drug Design
High-Throughput Nucleotide Sequencing / methods*
Humans
Sequence Analysis, DNA / methods*
Vaccines / chemistry*

Substances

Vaccines