Abstract
An EZ::TN<R6Kγori/KAN-2>Tnp transposon insertion in an open reading frame of unknown function (ncr) in Acinetobacter baumannii resulted in an 8-fold increase in ciprofloxacin resistance (Cip(r)). Transposon insertions in an ncr mutant that reduced Cip(r) back to wild type mapped to three genes encoding subunits of the RecCBD exonuclease. The ncr mutation increased transcription of the recCBD genes, and overexpression of the recCBD genes in a wild-type background resulted in a 4-fold increase in Cip(r).
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acinetobacter baumannii / drug effects
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Acinetobacter baumannii / genetics*
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DNA Transposable Elements
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Drug Resistance, Multiple, Bacterial / drug effects
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Drug Resistance, Multiple, Bacterial / genetics*
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Exodeoxyribonuclease V / genetics*
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Fluoroquinolones / pharmacology*
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Gene Expression
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Gene Library
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Genes, Bacterial*
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Genetic Engineering
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Microbial Sensitivity Tests
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Mutation
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Open Reading Frames
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Protein Subunits / genetics
Substances
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DNA Transposable Elements
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Fluoroquinolones
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Protein Subunits
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Exodeoxyribonuclease V