Experimental definition of a clustered regularly interspaced short palindromic duplicon in Escherichia coli

Moran G Goren; Ido Yosef; Oren Auster; Udi Qimron

doi:10.1016/j.jmb.2012.06.037

Experimental definition of a clustered regularly interspaced short palindromic duplicon in Escherichia coli

J Mol Biol. 2012 Oct 12;423(1):14-6. doi: 10.1016/j.jmb.2012.06.037. Epub 2012 Jul 3.

Authors

Moran G Goren¹, Ido Yosef, Oren Auster, Udi Qimron

Affiliation

¹ Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.

PMID: 22771574
DOI: 10.1016/j.jmb.2012.06.037

Abstract

We analyzed sequences of newly inserted repeats in an Escherichia coli CRISPR (clustered regularly interspaced short palindromic repeats) array in vivo and showed that a base previously thought to belong to the repeat is actually derived from a protospacer. Based on further experimental results, we propose to use the term "duplicon" for a repeated sequence in a CRISPR array that serves as a template for a new duplicon. Our findings suggest the possibility of redrawing the borders between repeats, spacers, and protospacer adjacent motifs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Escherichia coli / genetics*
Inverted Repeat Sequences / genetics*
Molecular Sequence Data
Segmental Duplications, Genomic / genetics