Akap200 suppresses the effects of Dv-cbl expression in the Drosophila eye

Mol Cell Biochem. 2012 Oct;369(1-2):135-45. doi: 10.1007/s11010-012-1376-x. Epub 2012 Jul 8.

Abstract

The Drosophila melanogaster orthologue of the c-Cbl proto-oncogene acts to downregulate signalling from receptor tyrosine kinases by enhancing endocytosis of activated receptors. Expression of an analogue of the C-terminally truncated v-Cbl oncogene, Dv-cbl, in the developing Drosophila eye conversely leads to excess signalling and disruption to the well-ordered adult compound eye. Co-expression of activated Ras with Dv-cbl leads to a severe disruption of eye development. We have used a transposon-based inducible expression system to screen for molecules that can suppress the Dv-cbl phenotype and have identified an allele that upregulates the A-kinase anchoring protein, Akap200. Overexpression of Akap200 not only suppresses the phenotype caused by Dv-cbl expression, but also the severe disruption to eye development caused by the combined expression of Dv-cbl and activated Ras. Akap200 is also endogenously expressed in the developing Drosophila eye at a level that modulates the effects of excessive signalling caused by expression of Dv-cbl.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • A Kinase Anchor Proteins* / genetics
  • A Kinase Anchor Proteins* / metabolism
  • Animals
  • Drosophila Proteins* / genetics
  • Drosophila Proteins* / metabolism
  • Drosophila melanogaster
  • Eye / growth & development*
  • Eye / metabolism
  • Gene Expression Regulation, Developmental
  • Membrane Proteins* / genetics
  • Membrane Proteins* / metabolism
  • Proto-Oncogene Proteins c-cbl* / genetics
  • Proto-Oncogene Proteins c-cbl* / metabolism
  • Signal Transduction
  • ras Proteins* / genetics
  • ras Proteins* / metabolism

Substances

  • A Kinase Anchor Proteins
  • Akap200 protein, Drosophila
  • Drosophila Proteins
  • Membrane Proteins
  • Proto-Oncogene Proteins c-cbl
  • Ras85D protein, Drosophila
  • ras Proteins
  • Cbl protein, Drosophila