The effect of timing of embryonic progression on chromosomal abnormality

Fertil Steril. 2012 Oct;98(4):876-80. doi: 10.1016/j.fertnstert.2012.06.014. Epub 2012 Jul 11.

Abstract

Objective: To evaluate the relationship between aneuploidy and timing of blastocyst formation.

Design: Historical cohort study.

Setting: Private IVF clinic.

Patient(s): Ninety-four couples undergoing IVF treatment in combination with chromosomal screening of embryos. The mean maternal age was 39.2 years and average number of embryos per patient 5.3.

Intervention(s): A total of 530 embryos were biopsied on day 3 and underwent chromosome screening with microarray-based comparative genomic hybridization.

Main outcome measure(s): Effect of day of embryo blastulation and morphologic grade on aneuploidy rate.

Result(s): Day 5 morulas that progressed to blastocysts on day 6 were significantly less likely to be aneuploid (79.8%) than day 5 morulas that did not progress to blastocysts (92.9%). However, there was no significant difference in aneuploidy rates when embryos that became blastocysts on day 5 were directly compared with embryos that became blastocysts on day 6.

Conclusion(s): Delayed blastulation is not associated with increased aneuploidy rates, but absence of blastulation is associated with increased aneuploidy. Therefore, we conclude that when choosing a morula for transfer on day 5, there may be a benefit in waiting an extra day for the possibility of blastulation to occur.

MeSH terms

  • Adult
  • Aneuploidy
  • Blastocyst / cytology*
  • Blastocyst / physiology*
  • Chromosome Aberrations*
  • Cohort Studies
  • Embryonic Development / genetics
  • Female
  • Fertilization in Vitro / methods*
  • Genetic Testing / methods
  • Humans
  • Logistic Models
  • Middle Aged
  • Morula / cytology
  • Morula / physiology
  • Pregnancy
  • Pregnancy Rate
  • Preimplantation Diagnosis / methods*
  • Time Factors