Requirement of signal peptidase ComC and thiol-disulfide oxidoreductase DsbA for optimal cell surface display of pseudopilin ComGC in Staphylococcus aureus

Magdalena M van der Kooi-Pol; Ewoud Reilman; Mark J J B Sibbald; Yanka K Veenstra-Kyuchukova; Thijs R H M Kouwen; Girbe Buist; Jan Maarten van Dijl

doi:10.1128/AEM.01565-12

Requirement of signal peptidase ComC and thiol-disulfide oxidoreductase DsbA for optimal cell surface display of pseudopilin ComGC in Staphylococcus aureus

Appl Environ Microbiol. 2012 Oct;78(19):7124-7. doi: 10.1128/AEM.01565-12. Epub 2012 Jul 20.

Authors

Magdalena M van der Kooi-Pol¹, Ewoud Reilman, Mark J J B Sibbald, Yanka K Veenstra-Kyuchukova, Thijs R H M Kouwen, Girbe Buist, Jan Maarten van Dijl

Affiliation

¹ Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.

Abstract

Staphylococcus aureus is an important Gram-positive bacterial pathogen producing many secreted and cell surface-localized virulence factors. Here we report that the staphylococcal thiol-disulfide oxidoreductase DsbA is essential for stable biogenesis of the ComGC pseudopilin. The signal peptidase ComC is indispensable for ComGC maturation and optimal cell surface exposure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / metabolism*
Gene Expression
Membrane Proteins / metabolism*
Protein Disulfide Reductase (Glutathione) / metabolism*
Serine Endopeptidases / metabolism*
Staphylococcus aureus / enzymology*
Staphylococcus aureus / metabolism*

Substances

Bacterial Proteins
Membrane Proteins
Protein Disulfide Reductase (Glutathione)
Serine Endopeptidases
type I signal peptidase