An integrated chip for the high-throughput synthesis of transcription activator-like effectors

Zhao Wang; Juan Li; Huang Huang; Gancheng Wang; Mingjun Jiang; Shenyi Yin; Changhong Sun; Hanshuo Zhang; Fengfeng Zhuang; Jianzhong Jeff Xi

doi:10.1002/anie.201203597

An integrated chip for the high-throughput synthesis of transcription activator-like effectors

Angew Chem Int Ed Engl. 2012 Aug 20;51(34):8505-8. doi: 10.1002/anie.201203597. Epub 2012 Jul 23.

Authors

Zhao Wang¹, Juan Li, Huang Huang, Gancheng Wang, Mingjun Jiang, Shenyi Yin, Changhong Sun, Hanshuo Zhang, Fengfeng Zhuang, Jianzhong Jeff Xi

Affiliation

¹ Biomedical Engineering Department, College of Engineering, Peking University, Yan Nan Yuan 60, Beijing 100871, China.

PMID: 22821513
DOI: 10.1002/anie.201203597

Abstract

A TALE of two assays: Transcription activator-like effectors (TALEs) are programmable proteins that can specifically recognize a DNA sequence. Previous strategies for the synthesis of TALEs were complicated and time-consuming. The solid-phase synthesis strategy demonstrated here allows quick and simple purification of the ligation product.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

HeLa Cells
High-Throughput Screening Assays / methods*
Humans
Protein Engineering
Trans-Activators / chemical synthesis*
Trans-Activators / genetics
Trans-Activators / metabolism
Transcription Factors / chemical synthesis*
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

Trans-Activators
Transcription Factors