Abstract
We present a model system to demonstrate that the positioning of biomolecules (membrane proteins) in a nonnative, complex thin film environment can be regulated by the phase behavior of film components. Partial separation between an amphiphilic polymer and a lipid drives the protein to a fluid phase, mechanically more similar to a cellular bilayer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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1,2-Dipalmitoylphosphatidylcholine / analogs & derivatives*
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1,2-Dipalmitoylphosphatidylcholine / chemistry
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Fluorescent Dyes
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Lipid Bilayers / chemistry*
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Membrane Proteins / chemistry*
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Microscopy, Atomic Force
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Oxazoles / chemistry*
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Phase Transition
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Polymers / chemistry*
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Porins / chemistry*
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Succinimides
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Surface-Active Agents / chemistry
Substances
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Alexa Fluor 488 carboxylic acid succinimidyl ester
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Fluorescent Dyes
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Lipid Bilayers
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Membrane Proteins
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OmpF protein
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Oxazoles
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Polymers
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Porins
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Succinimides
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Surface-Active Agents
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1,2-Dipalmitoylphosphatidylcholine
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colfosceril palmitate