Pyrosequencing of the rabies virus glycoprotein gene to demonstrate absence of vaccine-associated rabies cases following oral vaccination

Paola De Benedictis; Cristian De Battisti; Sabrina Marciano; Franco Mutinelli; Ilaria Capua; Giovanni Cattoli

doi:10.1016/j.tvjl.2012.06.030

Pyrosequencing of the rabies virus glycoprotein gene to demonstrate absence of vaccine-associated rabies cases following oral vaccination

Vet J. 2013 Mar;195(3):388-90. doi: 10.1016/j.tvjl.2012.06.030. Epub 2012 Jul 28.

Authors

Paola De Benedictis¹, Cristian De Battisti, Sabrina Marciano, Franco Mutinelli, Ilaria Capua, Giovanni Cattoli

Affiliation

¹ National Reference Centre for Rabies and OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, Legnaro, Padova, Italy. [email protected]

PMID: 22841448
DOI: 10.1016/j.tvjl.2012.06.030

Abstract

Replication competent vaccines have been used successfully for the control of terrestrial rabies, mainly in wildlife; however, these vaccine strains occasionally may induce rabies. In this study, a pyrosequencing protocol for the rapid identification of vaccine-associated rabies viruses was applied to the 2008-2011 Italian epidemic. There was no evidence of vaccine-associated rabies cases following oral vaccination of foxes with the SAG2 and SADB19 vaccine strains.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Oral
Animals
Foxes*
Global Health
Glycoproteins / genetics
Glycoproteins / metabolism*
Peptide Fragments / genetics
Peptide Fragments / metabolism*
Rabies / prevention & control
Rabies / veterinary*
Rabies Vaccines / administration & dosage
Rabies Vaccines / adverse effects
Rabies Vaccines / immunology*
Vaccination
Viral Proteins / genetics
Viral Proteins / metabolism*

Substances

Glycoproteins
Peptide Fragments
Rabies Vaccines
Viral Proteins
rabies virus glycoprotein peptide