An outbreak of Klebsiella pneumoniae late-onset sepsis in a neonatal intensive care unit in Guatemala

Am J Infect Control. 2012 Aug;40(6):516-20. doi: 10.1016/j.ajic.2012.02.031.

Abstract

Background: Gram-negative bloodstream infections are an important cause of neonatal mortality. In October 2009, we investigated a Klebsiella spp outbreak in a neonatal intensive care unit in Guatemala.

Methods: Probable cases were defined as a Klebsiella spp isolated from blood in neonates aged <28 days in the neonatal intensive care unit between October 1 and November 10, 2009; confirmed cases were identified as Klebsiella pneumoniae. Clinical data were abstracted from medical charts. K pneumoniae isolates were genotyped by pulsed-field gel electrophoresis (PFGE) and tested for antimicrobial susceptibility. Infection control practices were inspected.

Results: There were 14 confirmed cases. The median age at onset of infection was 3 days (range, 2-8 days). Nine patients died (64%). K pneumoniae isolates were resistant to multiple antimicrobials. PFGE revealed 2 distinct clusters. Breaches in infection control procedures included inappropriate intravenous solution use and inadequate hand hygiene and contact precautions.

Conclusions: We report a K pneumoniae outbreak with high neonatal mortality in Guatemala. PFGE clustering suggested a common source possibly related to reuse of a single-use intravenous medication or solution. The risk for K pneumoniae bloodstream infections in neonates in low-resource settings where sharing of solutions is common needs to be emphasized.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Cross Infection / epidemiology*
  • Cross Infection / mortality
  • Disease Outbreaks*
  • Drug Contamination
  • Electrophoresis, Gel, Pulsed-Field
  • Female
  • Genotype
  • Guatemala / epidemiology
  • Humans
  • Infant, Newborn
  • Intensive Care Units, Neonatal*
  • Klebsiella Infections / epidemiology*
  • Klebsiella Infections / mortality
  • Klebsiella pneumoniae / classification
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / isolation & purification*
  • Male
  • Microbial Sensitivity Tests
  • Molecular Typing
  • Sepsis / epidemiology*
  • Sepsis / mortality
  • Survival Analysis

Substances

  • Anti-Bacterial Agents