Generating an iPSC bank for HLA-matched tissue transplantation based on known donor and recipient HLA types

Craig J Taylor; Sarah Peacock; Afzal N Chaudhry; J Andrew Bradley; Eleanor M Bolton

doi:10.1016/j.stem.2012.07.014

Generating an iPSC bank for HLA-matched tissue transplantation based on known donor and recipient HLA types

Cell Stem Cell. 2012 Aug 3;11(2):147-52. doi: 10.1016/j.stem.2012.07.014.

Authors

Craig J Taylor¹, Sarah Peacock, Afzal N Chaudhry, J Andrew Bradley, Eleanor M Bolton

Affiliation

¹ Histocompatibility and Immunogenetics Laboratory, Cambridge University Teaching Hospitals NHS Foundation Trust, Addenbrooke's Hospital, Cambridge, CB2 0QQ, UK. [email protected]

PMID: 22862941
DOI: 10.1016/j.stem.2012.07.014

Abstract

The likelihood for immunological rejection of Human Leukocyte Antigens (HLA)-mismatched induced pluripotent stem cells (iPSCs) limits their therapeutic potential. Here we show how a tissue bank from 150 selected homozygous HLA-typed volunteers could match 93% of the UK population with a minimal requirement for immunosuppression. Our model provides a practical approach for using existing HLA-typed samples to generate an iPSC stem cell bank that circumvents prospective typing of a large number of individuals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

HLA Antigens / immunology*
Histocompatibility Testing*
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / immunology*
Stem Cell Transplantation / methods*
Tissue Donors*
Transplantation, Homologous

Substances

HLA Antigens