Abstract
Isoflavones found in soybeans and soy products possess clinically relevant properties. However, the anti-allergic effect of isoflavones has been poorly studied. We examined the effects of isoflavones, genistein, daidzein, and equol, on the expression of the high-affinity immunoglobulin E (IgE) receptor, FcεRI, which plays a central role in IgE-mediated allergic response. Flow cytometric analysis showed that all of these isoflavones reduced the cell surface expression of FcεRI on mouse bone-marrow-derived mast cells and human basophilic KU812 cells. All isoflavones decreased the levels of the FcεRIα mRNA in the cells. Genistein reduced the mRNA expression of the β chain, and daidzein and equol downregulated that of the γ chain. The suppressive effects of isoflavones on FcεRI expression were unaffected by ICI 182,780, an estrogen receptor antagonist, suggesting that these effects were independent of estrogen receptors.
MeSH terms
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Animals
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Anti-Allergic Agents / pharmacology
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Basophils / drug effects
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Basophils / metabolism
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Cell Line / drug effects
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Equol / pharmacology
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Estradiol / analogs & derivatives
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Estradiol / pharmacology
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Estrogen Antagonists / pharmacology
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Fulvestrant
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Genistein / pharmacology
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Glycine max / chemistry
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Humans
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Immunoglobulin E / metabolism
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Isoflavones / pharmacology*
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Male
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Mast Cells / drug effects
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Mast Cells / metabolism
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Mice
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Mice, Inbred BALB C
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Mitogen-Activated Protein Kinase 1 / metabolism
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Mitogen-Activated Protein Kinase 3 / metabolism
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Phosphorylation / drug effects
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Receptors, Estrogen / metabolism*
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Receptors, IgE / genetics
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Receptors, IgE / metabolism*
Substances
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Anti-Allergic Agents
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Estrogen Antagonists
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FcepsilonRI alpha-chain, human
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Isoflavones
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Receptors, Estrogen
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Receptors, IgE
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Fulvestrant
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Immunoglobulin E
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Estradiol
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Equol
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daidzein
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Genistein
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MAPK1 protein, human
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3