A generic strategy for co-presentation of heparin-binding growth factors based on CVD polymerization

Macromol Rapid Commun. 2012 Sep 14;33(17):1459-65. doi: 10.1002/marc.201200343. Epub 2012 Aug 7.

Abstract

A multifunctional copolymer with both aldehyde and alkyne groups is synthesized by chemical vapor deposition (CVD) for orthogonal co-immobilization of biomolecules. Surface analytical methods including FTIR and XPS are used to confirm the surface modification. Heparin-binding growth factors [basic fibroblast growth factor (bFGF) in this study] can be immobilized through interaction with heparin, which was covalently attached to the CVD surface through an aldehyde-hydrazide reaction. In parallel, an alkyne-azide reaction is used to orthogonally co-immobilize an adhesion peptide as the second biomolecule.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aldehydes / chemistry
  • Alkynes / chemistry
  • Azides / chemistry
  • Fibroblast Growth Factor 2 / chemistry*
  • Fibroblast Growth Factor 2 / metabolism
  • Gases / chemistry
  • Heparin / chemistry*
  • Heparin / metabolism
  • Immobilized Proteins / chemistry
  • Immobilized Proteins / metabolism
  • Microscopy, Fluorescence
  • Polymerization
  • Polymers / chemistry*
  • Surface Properties

Substances

  • Aldehydes
  • Alkynes
  • Azides
  • Gases
  • Immobilized Proteins
  • Polymers
  • Fibroblast Growth Factor 2
  • Heparin