Intravascular atherosclerotic imaging with combined fluorescence and optical coherence tomography probe based on a double-clad fiber combiner

J Biomed Opt. 2012 Jul;17(7):070501. doi: 10.1117/1.JBO.17.7.070501.

Abstract

We developed a multimodality fluorescence and optical coherence tomography probe based on a double-clad fiber (DCF) combiner. The probe is composed of a DCF combiner, grin lens, and micromotor in the distal end. An integrated swept-source optical coherence tomography and fluorescence intensity imaging system was developed based on the combined probe for the early diagnoses of atherosclerosis. This system is capable of real-time data acquisition and processing as well as image display. For fluorescence imaging, the inflammation of atherosclerosis and necrotic core formed with the annexin V-conjugated Cy5.5 were imaged. Ex vivo imaging of New Zealand white rabbit arteries demonstrated the capability of the combined system.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiography / instrumentation*
  • Animals
  • Atherosclerosis / diagnosis*
  • Endovascular Procedures / instrumentation*
  • Equipment Design
  • Equipment Failure Analysis
  • Fiber Optic Technology / instrumentation*
  • Microscopy, Fluorescence / instrumentation*
  • Rabbits
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Subtraction Technique / instrumentation*
  • Tomography, Optical Coherence / instrumentation*
  • Transducers