Injectable citrate-based mussel-inspired tissue bioadhesives with high wet strength for sutureless wound closure

Biomaterials. 2012 Nov;33(32):7972-83. doi: 10.1016/j.biomaterials.2012.07.055. Epub 2012 Aug 16.

Abstract

The existing surgical adhesives are not ideal for wet tissue adhesion required in many surgeries such as those for internal organs. Developing surgical adhesives with strong wet tissue adhesion, controlled degradability and mechanical properties, and excellent biocompatibility has been a significant challenge. Herein, learning from nature, we report a one-step synthesis of a family of injectable citrate-based mussel-inspired bioadhesives (iCMBAs) for surgical use. Within the formulations investigated, iCMBAs showed 2.5-8.0 folds stronger wet tissue adhesion strength over the clinically used fibrin glue, demonstrated controlled degradability and tissue-like elastomeric mechanical properties, and exhibited excellent cyto/tissue-compatibility both in vitro and in vivo. iCMBAs were able to stop bleeding instantly and suturelessly, and close wounds (2 cm long×0.5 cm deep) created on the back of Sprague-Dawley rats, which is impossible when using existing gold standard, fibrin glue, due to its weak wet tissue adhesion strength. Equally important, the new bioadhesives facilitate wound healing, and are completely degraded and absorbed without eliciting significant inflammatory response. Our results support that iCMBA technology is highly translational and could have broad impact on surgeries where surgical tissue adhesives, sealants, and hemostatic agents are used.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bivalvia / chemistry*
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Citric Acid / administration & dosage
  • Citric Acid / chemistry*
  • Citric Acid / therapeutic use*
  • Citric Acid / toxicity
  • Cross-Linking Reagents / chemistry
  • Injections
  • Mice
  • NIH 3T3 Cells
  • Polymers / chemistry
  • Polymers / therapeutic use
  • Polymers / toxicity
  • Rats
  • Rats, Sprague-Dawley
  • Tensile Strength
  • Tissue Adhesives / administration & dosage
  • Tissue Adhesives / chemistry*
  • Tissue Adhesives / therapeutic use*
  • Tissue Adhesives / toxicity
  • Wound Healing / drug effects*

Substances

  • Cross-Linking Reagents
  • Polymers
  • Tissue Adhesives
  • Citric Acid