Abstract
Enucleated oocytes have the distinctive ability to reprogram somatic nuclei back to totipotency. Here, we investigate genome-scale DNA methylation patterns after nuclear transfer and compare them to the dynamics at fertilization. We identify specific targets for DNA demethylation after nuclear transfer, such as germline-associated promoters, as well as unique limitations that include certain repetitive element classes.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cytoplasm / physiology*
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DNA Methylation / physiology
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Embryo, Mammalian
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Embryonic Development* / physiology
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Female
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Inbred DBA
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Nuclear Transfer Techniques
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Oocytes / cytology*
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Organ Specificity
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Promoter Regions, Genetic / genetics
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Promoter Regions, Genetic / physiology
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Sperm-Ovum Interactions / physiology