Abstract
To rapidly identify carbapenemase producers in Enterobacteriaceae, we developed the Carba NP test. The test uses isolated bacterial colonies and is based on in vitro hydrolysis of a carbapenem, imipenem. It was 100% sensitive and specific compared with molecular-based techniques. This rapid (<2 hours), inexpensive technique may be implemented in any laboratory.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / chemistry
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Bacterial Proteins / biosynthesis*
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Bacteriological Techniques
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Carbapenems / chemistry
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Enterobacteriaceae / enzymology*
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Enterobacteriaceae / isolation & purification
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Enterobacteriaceae Infections / diagnosis*
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Enterobacteriaceae Infections / microbiology
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Humans
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Hydrolysis
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Sensitivity and Specificity
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beta-Lactamases / biosynthesis*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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beta-Lactamases
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carbapenemase