Enhanced recognition and neutralization of HIV-1 by antibody-derived CCR5-mimetic peptide variants

J Virol. 2012 Nov;86(22):12417-21. doi: 10.1128/JVI.00967-12. Epub 2012 Aug 29.

Abstract

A tyrosine-sulfated CCR5-mimetic peptide, CCR5mim1, inhibits HIV-1 infection more efficiently than sulfopeptides based on the CCR5 amino terminus. Here we characterized sulfopeptide chimeras of CCR5mim1 and the heavy-chain CDR3 of the antibody PG16. Two chimeras bound a range of envelope glycoproteins and neutralized HIV-1 more efficiently than CCR5mim1. An immunoadhesin form of one of these, CCR5mim2-Ig, synergized with CD4-Ig to neutralize HIV-1. These sulfopeptides are among the broadest and most potent CCR5-mimetic peptides described to date.

MeSH terms

  • Antibodies, Neutralizing / chemistry
  • CD4-Positive T-Lymphocytes / cytology
  • Epitopes / chemistry
  • Glycoproteins / chemistry
  • HEK293 Cells
  • HIV Infections / metabolism*
  • HIV-1 / metabolism*
  • Humans
  • Neutralization Tests
  • Peptides / chemistry
  • Protein Binding
  • Protein Structure, Tertiary
  • Receptors, CCR5 / chemistry
  • Receptors, CCR5 / metabolism*

Substances

  • Antibodies, Neutralizing
  • Epitopes
  • Glycoproteins
  • Peptides
  • Receptors, CCR5