The purpose of this research was to compare the activities of different dose of epimedium polysaccharide-propolis flavone adjuvant (EPA). The inactivated avian influenza (AI) and Newcastle disease (ND) vaccine containing three doses of EPA were prepared. In AI vaccine vaccination experiment, 300 14-day-old chickens were randomly divided into 6 groups and inoculated with three EPA-AI vaccines taking oil adjuvant (OA), non-adjuvant (NA) vaccines and physiological saline as controls, repeated at 28-day-old. The lymphocyte proliferation and serum antibody titer were determined. In ND vaccine vaccination experiment, 300 14-day-old chickens were grouped, treated with three EPA-ND vaccines, and determined same to AI vaccine vaccination experiment; at 42-day-old the chickens were challenged with NDV. On D(15) after challenged, the immune protective effect was observed. The results showed that EPA could significantly promote lymphocyte proliferation and enhance serum antibody titer against AI and ND, and reduce the morbidity of chickens challenged with NDV after vaccinated with ND vaccine, especially the effect of medium dose was better than that of non-adjuvant and oil adjuvant. These results indicated that EPA could enhance the immune effect of inactivated AI vaccine and ND vaccine and would be expected as a new-type adjuvant.
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