Light-regulated host-guest interaction as a new strategy for intracellular PEG-detachable polyplexes to facilitate nuclear entry

Wenyu Li; Youxiang Wang; Lina Chen; Zhixiong Huang; Qiaoling Hu; Jian Ji

doi:10.1039/c2cc34768g

Light-regulated host-guest interaction as a new strategy for intracellular PEG-detachable polyplexes to facilitate nuclear entry

Chem Commun (Camb). 2012 Oct 18;48(81):10126-8. doi: 10.1039/c2cc34768g.

Authors

Wenyu Li¹, Youxiang Wang, Lina Chen, Zhixiong Huang, Qiaoling Hu, Jian Ji

Affiliation

¹ MOE key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, PR China.

PMID: 22952013
DOI: 10.1039/c2cc34768g

Abstract

PEG-detachable polyplexes were constructed for the first time via host-guest interactions between β-cyclodextrin and azobenzene. The polyplexes had excellent colloidal stability and competition stability. Moreover, the intracellular light-regulated dePEGylation facilitated DNA release and nuclear entry, thus resulting in efficient transfection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Azo Compounds / chemistry*
Azo Compounds / metabolism
DNA / administration & dosage*
DNA / genetics
Drug Carriers / chemistry*
Drug Carriers / metabolism
HEK293 Cells
Hep G2 Cells
Humans
Light
Polyethylene Glycols / chemistry*
Polyethylene Glycols / metabolism
Transfection*
beta-Cyclodextrins / chemistry*
beta-Cyclodextrins / metabolism

Substances

Azo Compounds
Drug Carriers
beta-Cyclodextrins
Polyethylene Glycols
DNA
azobenzene
betadex