Performance of the Xpert® MTB/RIF assay in an active case-finding strategy: a pilot study from Tanzania

Int J Tuberc Lung Dis. 2012 Nov;16(11):1468-70. doi: 10.5588/ijtld.12.0127. Epub 2012 Sep 7.

Abstract

In this pilot study, we evaluated the Xpert® MTB/RIF assay in an active case-finding strategy, using two spot sputum samples collected within a 1-hour interval from household contacts of smear-positive TB index cases. Tuberculosis (TB) confirmed by culture served as the reference standard. Among 219 enrolled contacts, the yield of active TB was 2.3%. While the sensitivity of smear microscopy was 60% (95%CI 14.7-94.7), Xpert MTB/RIF achieved a sensitivity of 100% (95%CI 47.81-100.0). All culture-confirmed cases tested positive by Xpert MTB/RIF on the first submitted sample, suggesting that the evaluation of only one sample could be sufficient for TB diagnosis in this context.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Child, Preschool
  • Contact Tracing / methods*
  • Cross-Sectional Studies
  • Feasibility Studies
  • Female
  • Humans
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis / isolation & purification*
  • Nucleic Acid Amplification Techniques / methods*
  • Pilot Projects
  • Sensitivity and Specificity
  • Sputum / microbiology
  • Tanzania / epidemiology
  • Tuberculosis / diagnosis*
  • Tuberculosis / epidemiology
  • Tuberculosis / microbiology
  • Young Adult