Low-density lipoprotein receptor mutations generate synthetic genome-wide associations

Eur J Hum Genet. 2013 May;21(5):563-6. doi: 10.1038/ejhg.2012.207. Epub 2012 Sep 12.

Abstract

Genome-wide association (GWA) studies have discovered multiple common genetic risk variants related to common diseases. It has been proposed that a number of these signals of common polymorphisms are based on synthetic associations that are generated by rare causative variants. We investigated if mutations in the low-density lipoprotein receptor (LDLR) gene causing familial hypercholesterolemia (FH, OMIM #143890) produce such signals. We genotyped 480 254 polymorphisms in 464 FH patients and in 5945 subjects from the general population. A total of 28 polymorphisms located up to 2.4 Mb from the LDLR gene were genome-wide significantly associated with FH (P<10(-8)). We replicated the 10 top signals in 2189 patients with a clinical diagnosis of FH and in 2157 subjects of a second sample of the general population (P<0.000087). Our findings confirm that rare variants are able to cause synthetic genome-wide significant associations, and that they exert this effect at relatively large distances from the causal mutation.

MeSH terms

  • Cohort Studies
  • Genome-Wide Association Study
  • Genotype
  • Haplotypes / genetics
  • Humans
  • Hyperlipoproteinemia Type II / genetics*
  • Mutation / genetics
  • Oligonucleotide Array Sequence Analysis
  • Polymorphism, Single Nucleotide / genetics*
  • Receptors, LDL / genetics*

Substances

  • Receptors, LDL