Glulathione-S-transferases gene polymorphism in prediction of gastric cancer risk by smoking and Helicobacter pylori infection status

Asian Pac J Cancer Prev. 2012;13(7):3325-8. doi: 10.7314/apjcp.2012.13.7.3325.

Abstract

Aim: To evaluate the association of glutathione S-transferases gene polymorphisms with the risk of gastric cancer, with reference to smoking and Helicobacter pylori infection.

Methods: We conducted a 1:1 matched case-control study with 410 gastric cancer cases and 410 cancer-free controls. Polymorphisms of GSTM1, GSTT1 and GSTP1 were determined using PCR-CTPP.

Results: The GSTM1 and GSTT1 null genotypes were significantly associated with the risk of gastric cancer after adjusting for potential confounding factors (OR=1.68, 95% CI=1.32-2.23 for null GSTM1, OR=1.73; 95% CI=1.24-2.13 for null GSTT1). The combination of null GSTM1 and null GSTT1 conferred an elevated risk (OR=2.54, 95% CI=1.55-3.39). However, no association was found for GSTP1 polymorphism The smoking modified the association of GSTM1 and GSTT1 null genotypes with the risk of gastric cancer.

Conclusion: GSTM1 and GSTT1 null genotypes are associated with increased risk of gastric cancer, and smoking modifies the association.

MeSH terms

  • Aged
  • Case-Control Studies
  • Female
  • Genetic Predisposition to Disease
  • Genotype
  • Glutathione S-Transferase pi / genetics
  • Glutathione Transferase / genetics*
  • Helicobacter Infections / complications
  • Helicobacter Infections / enzymology
  • Helicobacter Infections / genetics*
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Male
  • Polymorphism, Genetic
  • Risk
  • Smoking / adverse effects
  • Smoking / genetics*
  • Stomach Neoplasms / enzymology
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / microbiology*

Substances

  • glutathione S-transferase T1
  • GSTP1 protein, human
  • Glutathione S-Transferase pi
  • Glutathione Transferase
  • glutathione S-transferase M1