Conjugation of transferrin to azide-modified CdSe/ZnS core-shell quantum dots using cyclooctyne click chemistry

Angew Chem Int Ed Engl. 2012 Oct 15;51(42):10523-7. doi: 10.1002/anie.201202876. Epub 2012 Sep 20.

Abstract

Twinkle twinkle quantum dot: Conjugation of biomolecules to azide-modified quantum dots (QDs) through a bifunctional linker, using strain-promoted azide-alkyne cycloaddition with the QD and a squaramide linkage to the biomolecule (see scheme). Transferrin-conjugated QDs were internalized by transferrin-receptor expressing HeLa cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / chemistry*
  • Azides / chemistry*
  • Cadmium Compounds / chemistry*
  • Click Chemistry / methods
  • Cyclooctanes / chemical synthesis
  • Cyclooctanes / chemistry*
  • HeLa Cells
  • Humans
  • Models, Molecular
  • Molecular Structure
  • Quantum Dots*
  • Selenium Compounds / chemistry*
  • Sulfides / chemistry*
  • Transferrin / chemistry*
  • Zinc Compounds / chemistry*

Substances

  • Alkynes
  • Azides
  • Cadmium Compounds
  • Cyclooctanes
  • Selenium Compounds
  • Sulfides
  • Transferrin
  • Zinc Compounds
  • cadmium selenide
  • zinc sulfide