Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts

K R Harkins; R A Jefferson; T A Kavanagh; M W Bevan; D W Galbraith

doi:10.1073/pnas.87.2.816

Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts

Proc Natl Acad Sci U S A. 1990 Jan;87(2):816-20. doi: 10.1073/pnas.87.2.816.

Authors

K R Harkins¹, R A Jefferson, T A Kavanagh, M W Bevan, D W Galbraith

Affiliation

¹ Department of Plant Sciences, University of Arizona, Tucson 85721.

Abstract

We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Sequence
Chimera
Cloning, Molecular*
DNA / genetics
Flow Cytometry
Gene Expression / drug effects
Genes*
Herbicides / pharmacology
Molecular Sequence Data
Mosaic Viruses
Nicotiana / genetics
Photosynthesis*
Plants / genetics*
Plants, Toxic
Protoplasts / metabolism
Pyridazines / pharmacology
Transcription, Genetic
Transfection*

Substances

Herbicides
Pyridazines
DNA
norflurazone