Role of hyaluronic acid, its degrading enzymes, degradation products, and ferritin in the assessment of fibrosis stage in Egyptian patients with chronic hepatitis C

Eur J Gastroenterol Hepatol. 2013 Jan;25(1):69-76. doi: 10.1097/MEG.0b013e3283594924.

Abstract

Background/aims: Liver biopsy is considered a gold standard for fibrosis staging, but it has a high risk of morbidity. Therefore, there is an interest in developing noninvasive markers for the prediction of liver fibrosis stages.

Methods: Hyaluronic acid, ferritin, N-acetyl-β-D-glucosaminidase, β-glucuronidase, glucosamine, aspartate transaminase, and alanine transaminase were assayed in 210 individuals with chronic hepatitis C infection. Statistical analysis was carried out by logistic regression and receiver-operating characteristic curves.

Results: The best linear combination of only significant blood markers was used for the determination of the fibrosis discriminant score; score=[1.64 (numerical constant)-0.002×hyaluronic acid (pg/l)-2.68×β-glucuronidase (µmol/ml/min)-0.026×glucosamine (µg/dl)-0.001×ferritin-0.033 (ng/ml)×aspartate transaminase/alanine transaminase]. The selected fibrosis discriminant score function correctly classified 81% of patients with severe liver fibrosis at a discriminant cut-off score=0.55 (i.e. less than 0.55 indicated mild liver fibrosis and greater than 0.55 indicated severe liver fibrosis), with a sensitivity of 100% and a specificity of 73%.

Conclusion: A simple fibrosis index can be useful to select hepatitis C virus-infected patients with a very low risk of significant fibrosis in whom the protocol of liver biopsies may be avoided.

MeSH terms

  • Acetylglucosaminidase / blood
  • Adult
  • Alanine Transaminase / blood
  • Aspartate Aminotransferases / blood
  • Biomarkers / blood
  • Biopsy
  • Decision Support Techniques
  • Discriminant Analysis
  • Egypt / epidemiology
  • Female
  • Ferritins / blood*
  • Glucosamine / blood*
  • Glucuronidase / blood*
  • Hepatitis C, Chronic / blood
  • Hepatitis C, Chronic / complications
  • Hepatitis C, Chronic / diagnosis*
  • Humans
  • Hyaluronic Acid / blood*
  • Hyaluronoglucosaminidase / blood
  • Linear Models
  • Liver Cirrhosis / blood
  • Liver Cirrhosis / diagnosis*
  • Liver Cirrhosis / virology
  • Logistic Models
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Predictive Value of Tests
  • ROC Curve
  • Risk Assessment
  • Risk Factors
  • Sensitivity and Specificity
  • Severity of Illness Index
  • Unnecessary Procedures

Substances

  • Biomarkers
  • Hyaluronic Acid
  • Ferritins
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • Glucuronidase
  • Hyaluronoglucosaminidase
  • Acetylglucosaminidase
  • Glucosamine